Abstract: Mechanical Isolation of Adipose-Derived Stromal Vascular Fraction: Is It Becoming a Growing Trend?
نویسندگان
چکیده
MaterialS and MethodS: Adults undergoing liposuction on bilateral body areas were included (n=12). Under sedation, liposuction on one side was conducted with standard tumescent (1L of LR with 30ml of 1% lidocaine and 1 mcg/ml epinephrine). Tumescent without lidocaine was infiltrated on the contralateral side. Five milliliter lipoaspirate samples were processed for isolation of the stromal vascular fraction (SVF). Apoptosis and necrosis of SVF were examined by Annexin V-FITC/PI staining and analyzed by flow cytometry. ASC’s were also cultured and, after 24h, adherent, viable ASC’s were counted. The effect of ropivacaine on ASC survival was compared to PBS control and lidocaine in a cell culture, dose-response model.
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